Material and Safety Data Sheet
Fascin (human, recombinant) - 2x50 µg
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Fascin
Actin-bundling protein; human fascin 1
Applications
| Recommended use | Fascin is suited for in vitro actin network reconstitution, filament cross-linking and bundling assays, fluorescence microscopy-based actin-bundling experiments, and studies of cytoskeleton remodeling where controlled formation of actin bundles is required. |
Description
| Protein | Fascin 1 |
| Synonyms | Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55 |
| Origin | Human recombinant, untagged |
| Molecular mass | 55 kDa |
| Protein description | Fascin is a monomeric actin-bundling protein with a molecular mass of 55 kDa. Fascin-mediated bundling of actin filaments contributes to the formation of filopodial bundles that push the plasma membrane forward during cell movement, migration and cytoskeleton remodeling. Fascin has also been associated with the assembly of microspikes, stress fibers and membrane ruffles. |
| Actin interaction | Ca++-insensitive actin-bundling protein. |
Properties
| Form | Lyophilized protein, ready-to-use after reconstitution. |
| Quantity per unit | 2 × 50 µg |
| Buffer (after reconstitution) | 150 mM NaCl, 20 mM HEPES pH 7.4, 1 mM DTT and 5% sucrose after reconstitution with 50 µl ultrapure water to obtain a 1.0 mg/ml solution. |
| Purity | >99% by scanning densitometry of Coomassie G-250 stained SDS-PAGE. |
| Activity control | Bundling activity was verified by fluorescence microscopy using ATTO565-Actin and fascin at defined molar ratios. In low-speed co-sedimentation assays with rabbit skeletal muscle actin, fascin co-pelleted with F-actin under the tested assay conditions. |
| Purification notes | Human fascin was amplified from human macrophage cDNA and expressed as a GST-tag fusion protein in E. coli. After purification by GST-agarose and GST-tag cleavage, the untagged protein was purified by gel-filtration chromatography. |
| Protein concentration | Determined by OD280 (0.1% = 1.224). |
| Storage instructions | Store at −70°C upon arrival and use within the indicated stability period from date of purchase. Keep the solubilized protein on ice during use. For storage after reconstitution, prepare glycerol stocks or use CryoProtect and store at −20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. Upon receipt store at −70°C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are prepared with ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product Data Sheet
Material and Safety Data Sheet
Protein Sequence on NCBI
Protein Structure on RCSB PDB
Selected References
Reconstitution of contractile actomyosin rings in vesicles.
Nat Commun. 2021;12:2254.
Structure, evolutionary conservation, and conformational dynamics of Homo sapiens fascin-1, an F-actin crosslinking protein.
J Mol Biol. 2010;400:589.
Product DataSheetProtein Sequence on NCBIProtein Sequences and Structures on RCSB
Material and Safety Data SheetReconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P.Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
J.Mol.Biol. (2010) 400 p.589
