MonoMix .1
G-Actin buffer with ATP and 0.1mM DTT | 5x 50ml lyophilized
Cat. #: 8110-01
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Applications
G-actin buffer for applications sensitive to reducing agents. Used to dilute G-actin or G-actin ligands.
Description
Product name |
MonoMix .1 |
Product description |
MonoMix .1 is a G-actin buffer with reduced DTT concentration compared to MonoMix (Cat. #5100-01), containing 0.1mM DTT. This buffer is used to dilute G-actin or G-actin ligands.
Actin from Hypermol® is supplied in MonoMix and can be easily reconstituted by adding ultrapure water. For further dilution of G-actin, or G-actin ligands, MonoMix .1 is used. |
Overview |
Essential G-actin buffer to:
- Maintain the G-actin state.
- Used for dialysis of actin and ligands in actin assays.
- Support assays sensitive to DTT.
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Properties
Form |
Lyophilized, ready-to-use. |
Quantity |
5x1 ml 50x stock (reconstitute each tube with 1 ml ultrapure water to obtain a 50x stock).
The stocks can be diluted to prepare a total of 250 ml of G-actin buffer. This buffer is ideal for dialyzing G-actin or actin ligands. |
Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.1mM CaCl2 and 0.1mM DTT, when each tube is reconstituted with 50 ml ultrapure water. |
Purity |
Ultrapure reagents. |
Storage instructions |
MonoMix .1 is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized buffer is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
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Further Information
Product DataSheet
Material and Safety Data Sheet
Buffers from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
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