MonoMix - 100 ml (G-Actin buffer with 1mM DTT)
MonoMix
G-actin buffer with ATP; 100ml (2 × 50 ml) supplied as lyophilized 50× stock.
Applications
| Applications | G-actin buffer for dilution, dialysis and handling of G-actin or G-actin-binding ligands under defined actin-compatible buffer conditions. |
| Recommended use | MonoMix is used when G-actin or ligands need to be diluted or equilibrated into low-ionic-strength buffer conditions before actin polymerization assays, ligand-binding assays or other actin-based biochemical workflows. |
Description
| Product name | MonoMix |
| Product description | MonoMix is a defined G-actin buffer concentrate containing ATP, CaCl2 and DTT for standardized handling of monomeric actin. The buffer composition is selected to provide actin-compatible conditions during reconstitution, dilution and dialysis steps. ATP and Ca2+ support the nucleotide- and divalent-cation conditions typically required for stable G-actin handling. DTT provides reducing conditions during short-term use after reconstitution. MonoMix is therefore suitable for preparing G-actin samples or ligand samples before controlled initiation of polymerization with an appropriate polymerization buffer. |
| Overview | Defined G-actin-compatible buffer system; supplied as lyophilized 50× stock; convenient preparation of reproducible 1× working buffer; suitable for dilution and dialysis of actin and actin ligands. |
| Related workflow | MonoMix is intended for G-actin-compatible dilution and equilibration steps. For initiation of actin polymerization in the absence of nucleators, PolyMix may be used as the corresponding polymerization buffer. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution. |
| Quantity per unit | 2 × 1.0 ml 50× stock after reconstitution. Each lyophilized tube is reconstituted with 1.0 ml ultrapure water to obtain a 50× stock solution. Each 1.0 ml 50× stock tube is sufficient to prepare 50 ml of 1× G-actin buffer. Total 1× buffer volume per unit: 2 × 50 ml = 100 ml. |
| Buffer | Final 1× buffer composition: 2 mM Tris-HCl pH 8.2, 0.4 mM ATP, 0.1 mM CaCl2 and 1 mM DTT. |
| Reconstitution | Reconstitute each lyophilized tube with 1.0 ml ultrapure water to obtain the 50× stock. Dilute the stock 1:50 with ultrapure water to prepare the 1× working buffer. |
| Purity | Prepared from ultrapure reagents. |
| Storage instructions | MonoMix stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized buffer is kept on ice and should be used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
