Goat Anti-Donkey IgG ATTO594 (H+L)
Antibody produced in goat, affinity purified
Cat. #: 3909-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Donkey IgG ATTO594 (H+L) | GXD ATTO594 |
| Target species | Donkey |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Goat Anti-Donkey IgG ATTO594 (H+L) is optimized for high-resolution fluorescence microscopy, providing brilliant and stable signals for demanding imaging conditions. ATTO594 exhibits a distinct excitation/emission spectrum (λex: 601 nm, λem: 627 nm), making it an excellent choice for imaging systems requiring far-red detection. Its high photostability and low autofluorescence are particularly advantageous for live-cell imaging and thick tissue samples, minimizing background interference and enabling deep tissue penetration. This antibody is preservative-free, ensuring compatibility with sensitive applications. Its low non-specific binding and high affinity allow accurate target detection, while its robust fluorescence properties facilitate consistent results in advanced techniques such as flow cytometry, super-resolution microscopy, and fluorescence resonance energy transfer (FRET) assays. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 601 nm, λem: 627 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Donkey IgG ATTO594 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to donkey IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
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Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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