Goat Anti-Donkey IgG ATTO647N (H+L)
Antibody produced in goat, affinity purified
Cat. #: 3910-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Donkey IgG ATTO647N (H+L) | GXD ATTO647N |
| Target species | Donkey |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Goat Anti-Donkey IgG ATTO647N (H+L) is highly regarded as a gold standard for far-red fluorescence detection in advanced imaging. ATTO647N exhibits a distinct excitation/emission spectrum (λex: 646 nm, λem: 664 nm), offering remarkable brightness and photostability. Its far-red emission minimizes autofluorescence and enhances imaging depth, making it particularly effective for thick tissue samples and live-cell imaging. The dye's low background fluorescence ensures superior contrast and signal clarity, critical for super-resolution microscopy and single-molecule studies. This antibody is preservative-free, ensuring compatibility with sensitive applications. Its low non-specific binding and high affinity allow accurate target detection, while its robust fluorescence properties support consistent results in techniques such as flow cytometry, confocal microscopy, and fluorescence resonance energy transfer (FRET) assays. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 646 nm, λem: 664 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Donkey IgG ATTO647N (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to donkey IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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