Goat Anti-Guinea Pig IgG (H+L) ATTO590
Antibody produced in goat, affinity purified & preservative-free.
Cat. #:2721-1MG
Applications
Optimized for fluorescence microscopy, flow cytometry, and other fluorescence-based applications; preservative-free for compatibility with sensitive assays.
Description
| Product name | Goat Anti-Guinea Pig IgG (H+L) ATTO590 | GXGP ATTO590 |
| Target species | Guinea Pig |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence brightness with strong photostability for robust performance in demanding fluorescence applications. Goat Anti-Guinea Pig IgG (H+L) ATTO590 is designed for high-sensitivity detection and high-resolution imaging with low background. ATTO 590 features an excitation/emission spectrum (λex: 593 nm, λem: 622 nm) that is well suited for orange-red detection channels and multiplexing workflows. Typical excitation sources include 561 nm or 594 nm laser lines. The antibody is preservative-free and supplied to support sensitive fluorescence workflows, including advanced microscopy and quantitative immunofluorescence, while minimizing non-specific binding and background signal. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 593 nm, λem: 622 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20. |
| Content | 1.0 mg Goat Anti-Guinea Pig IgG (H+L) ATTO590 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to mouse IgG. After dye conjugation, the antibody was further purified by gel filtration to remove unconjugated dye and aggregates, minimizing background fluorescence. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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