Goat Anti-Guinea Pig IgG (H+L) ATTO633
Antibody produced in goat, affinity purified
Cat. #: 2722-1MG
Applications
Optimized for confocal microscopy, widefield fluorescence imaging, and flow cytometry; preservative-free for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Guinea Pig IgG (H+L) ATTO633 | GXGP ATTO633 |
| Target species | Guinea Pig |
| Description |
ATTO-labeled secondary antibodies are designed for high-performance fluorescence detection, combining strong signal intensity with excellent photostability. Goat Anti-Guinea Pig IgG (H+L) ATTO633 provides reliable, low-background labeling for fluorescence workflows requiring sensitive detection and stable far-red emission. ATTO 633 is a far-red fluorophore with an excitation/emission spectrum (λex: 630 nm, λem: 651 nm) that fits common 633 nm laser lines (e.g., He-Ne or diode lasers) and supports multiplex imaging with reduced autofluorescence and low spectral overlap. The antibody is supplied preservative-free for compatibility with demanding assays and advanced microscopy applications. Consistent conjugate quality and low levels of free dye help minimize background fluorescence and improve quantitative performance in imaging and flow cytometry. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 630 nm, λem: 651 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20. |
| Content | 1.0 mg Goat Anti-Guinea Pig IgG (H+L) ATTO633 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to guinea pig IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
Validated product quality with Satisfaction Guarantee!
