Goat Anti-Mouse IgG ATTO590 (H+L)
Affinity purified goat secondary antibody; preservative-free.
Cat. #: 2121-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Mouse IgG ATTO590 (H+L) | GXM ATTO590 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Goat Anti-Mouse IgG ATTO590 (H+L) is optimized for high-resolution fluorescence microscopy, providing brilliant and stable signals for demanding imaging conditions. ATTO590 supports robust red-channel detection. Its high photostability and low background contribution (low autofluorescence) are advantageous for live-cell imaging and thick tissue specimens, where reduced nonspecific signal improves contrast and supports deeper optical interrogation. Accordingly, ATTO590 is well suited for demanding applications including single-molecule detection and high-resolution fluorescence microscopy (e.g., PALM, dSTORM, STED). The dye is also highly compatible with flow cytometry (FACS) and fluorescence in situ hybridization (FISH), among other fluorescence-based assays. Fluorescence excitation is most efficient in the 575–610 nm range. This antibody is preservative-free, ensuring compatibility with sensitive applications. Its low non-specific binding and high affinity allow accurate target detection, while its robust fluorescence properties facilitate consistent results in advanced techniques such as flow cytometry, super-resolution microscopy, and fluorescence resonance energy transfer (FRET) assays. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 593 nm, λem: 622 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Mouse IgG ATTO590 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to mouse IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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