Goat Anti-Mouse IgG (H+L) ATTO700
Antibody produced in goat, affinity purified
Cat. #: 2110-1MG
Applications
Optimized for near-infrared imaging, offering minimal background fluorescence and compatibility with multiplexing techniques in advanced microscopy and flow cytometry.
Description
| Product name | Goat Anti-Mouse IgG (H+L) ATTO700 | GXM ATTO700 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies represent a cutting-edge advancement in fluorescence imaging, characterized by their exceptional photostability, high fluorescence quantum yield, and minimal photobleaching. Goat Anti-Mouse IgG ATTO700 is designed for near-infrared imaging, offering a distinct excitation/emission spectrum that ensures low background fluorescence and optimal signal-to-noise ratio, especially in thick or complex biological samples. The ATTO700 dye features an excitation/emission spectrum (λex: 700 nm, λem: 720 nm), making it particularly suited for applications requiring deep tissue penetration and reduced light scattering. Its near-infrared properties are ideal for multiplex imaging and live-cell microscopy, minimizing spectral overlap and enabling precise target localization in demanding experimental conditions. This antibody is preservative-free, making it compatible with sensitive applications. Its robust performance in fluorescence resonance energy transfer (FRET) assays, flow cytometry, and super-resolution microscopy underscores its versatility and reliability for advanced research needs. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 700 nm, λem: 720 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) Affinity purification effectively removed all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO490LS the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. |
| Content | 1.0 mg Goat Anti-Mouse IgG (H+L) ATTO700 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO 700 the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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