Goat Anti-Mouse IgG (H+L) ATTO700
Antibody produced in goat
Cat. # 2110-1MG
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Description
Product name
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Goat Anti-Mouse IgG ATTO700 (H+L)
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Target species
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Mouse
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Description
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ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG ATTO700 (H+L) is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield. The antibody is suited for all applications, including live cell techniques, since it contains no preservatives.
For convenient reference, the Fluorescent Antibody Kits contain gratis a primary epitope mapped actin antibody of high performance also on western blots.
Goat Anti-Mouse IgG ATTO700 is supplied in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. For convenient reconstitution, a glycerol buffer is provided. This enables re-freezing of the antibody at -20°C after use to maintain quality and allows for the preparation of aliquots, which can also be stored at -20°C for sequential use.
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Specification
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Goat anti-mouse IgG ATTO700 (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
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Optical Properties
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λex 700nm, λem 719nm
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Properties
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Form
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Lyophilized
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Nominal concentration
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2 mg/ml (after reconstitution with buffer)
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Content
Buffer
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1 mg Goat Anti-Mouse IgG (H+L) ATTO700
20µg (1mg/ml) Monoclonal Anti-Actin - for reference
1 ml Glycerol buffer (add 0.5ml to reconstitute the lyophilized antibody)
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.
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Clonality
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Polyclonal
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Isotype
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IgG
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Purification notes
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Column affinity purified.
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Storage instructions
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Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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Shipped at ambient temperature.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.