Goat Anti-Mouse IgM (H+L) ATTO390
Antibody produced in goat, affinity purified
Cat. #: 2201-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Mouse IgM (H+L) ATTO390 | GXM IgM ATTO390 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Goat Anti-Mouse IgM ATTO390 is specifically optimized for high-resolution microscopy, offering remarkable photostability and a high fluorescence quantum yield. These properties enable prolonged imaging sessions with minimal photobleaching, ensuring sharp and reliable signals. The ATTO390 dye exhibits a distinct excitation/emission spectrum (390/479 nm), providing excellent compatibility with UV-excitable systems and minimizing spectral overlap in multiplex imaging setups. Its strong fluorescence signal and low autofluorescence background make it highly effective in detecting low-abundance targets and achieving precise spatial resolution, even in dense or complex biological samples. This antibody is preservative-free, making it suitable for live-cell imaging and other sensitive applications requiring minimal interference. Its low non-specific binding and high affinity ensure accurate target detection, while its robust fluorescence properties allow consistent results in advanced techniques such as super-resolution microscopy, flow cytometry, and fluorescence resonance energy transfer (FRET) assays. |
| Specification | Immunofluorescence: 1:300–1:1000, Degree of Labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 390 nm, λem: 479 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Mouse IgM (H+L) ATTO390 20 µg Monoclonal Anti-Actin (1 mg/ml) - for reference 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgM |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to mouse IgM. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
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Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
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J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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