Fluorescent Antibody Kit
Goat Anti-Mouse IgM (H+L) ATTO490LS
Antibody produced in goat
Cat. # 2209-1MG
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Description
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Product name
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Goat Anti-Mouse IgM ATTO490LS (H+L)
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Target species
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Mouse
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Description
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ATTO-labeled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgM ATTO490LS has a very large stokes shift (165nm). It is thus is specifically suited for suited for all multiplexing approaches, especially in combination with ATTO488 and ATTO514 conjugated antibodies.
For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope mapped actin antibody of high performance also on western blots.
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Specification
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Goat Anti-Mouse IgM ATTO490 LS (H+L), Immunofluorescence: 1:300-1:1000, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
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Optical Properties
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λex 496nm, λem 661nm
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Properties
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Form
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Lyophilized.
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Nominal concentration
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2 mg/ml
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Content
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1 mg Goat Anti-Mouse IgM (H+L) ATTO490LS
20 µg (1mg/ml) Monoclonal Anti-Actin
1 ml Glycerol buffer (to reconstitute the antibody)
Buffer:
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 10 mM sodium azide, pH 7.4.
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Clonality
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Polyclonal
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Isotype
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IgM
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Purification notes
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Column affinity purified.
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Storage instructions
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Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
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Shipping conditions
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Shipped at ambient temperature.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.