Goat Anti-Rabbit IgG (H+L) ATTO488
Antibody produced in goat, affinity purified
Cat. #: 2302-1MG
Applications
Suited for high-sensitivity fluorescence microscopy, flow cytometry, and other fluorescence-based applications, containing no preservatives for compatibility with sensitive assays.
Description
| Product name | Goat Anti-Rabbit IgG (H+L) ATTO488 | GXR ATTO488 |
| Target species | Rabbit |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their high fluorescence quantum yield, superior photostability, and consistent performance across various applications. Goat Anti-Rabbit IgG ATTO488 is optimized for applications demanding high sensitivity and resolution. The ATTO488 dye exhibits an excitation/emission spectrum (λex: 501 nm, λem: 523 nm) that ensures bright green fluorescence and minimal spectral overlap. Its exceptional fluorescence intensity and low background autofluorescence enable the detection of low-abundance targets with precision, even in complex biological samples. This antibody is preservative-free, making it compatible with live-cell imaging, fluorescence resonance energy transfer (FRET), and super-resolution microscopy. Its enhanced photostability ensures reliable results during prolonged imaging sessions, making it an excellent choice for advanced fluorescence-based techniques. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 501 nm, λem: 523 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Rabbit IgG (H+L) ATTO488 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
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Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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