Goat Anti-Rabbit IgG (H+L) ATTO488
Antibody produced in goat, affinity purified
Cat. #: 2302-1MG
Applications
Suited for high-sensitivity fluorescence microscopy, flow cytometry, and other fluorescence-based applications, containing no preservatives for compatibility with sensitive assays.
Description
| Product name | Goat Anti-Rabbit IgG (H+L) ATTO488 | GXR ATTO488 |
| Target species | Rabbit |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their high fluorescence quantum yield, superior photostability, and consistent performance across various applications. Goat Anti-Rabbit IgG ATTO488 is optimized for applications demanding high sensitivity and resolution. The ATTO488 dye exhibits an excitation/emission spectrum (λex: 501 nm, λem: 523 nm) that ensures bright green fluorescence and minimal spectral overlap. Its exceptional fluorescence intensity and low background autofluorescence enable the detection of low-abundance targets with precision, even in complex biological samples. This antibody is preservative-free, making it compatible with live-cell imaging, fluorescence resonance energy transfer (FRET), and super-resolution microscopy. Its enhanced photostability ensures reliable results during prolonged imaging sessions, making it an excellent choice for advanced fluorescence-based techniques. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 501 nm, λem: 523 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Rabbit IgG (H+L) ATTO488 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to rabbit IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
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Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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