
Goat Anti-Rabbit IgG ATTO490LS (H+L)
Antibody produced in goat, affinity purified
Cat. #: 2309-1MG
Applications
Suited for fluorescence microscopy, flow cytometry, and other fluorescence-based applications, containing no preservatives for compatibility with sensitive assays.
Description
Product name |
Goat Anti-Rabbit IgG (H+L) ATTO490LS | GRX ATTO490LS |
Target species |
Rabbit |
Description |
ATTO-labeled secondary antibodies represent an advanced generation of fluorescent reagents, combining exceptional brightness, superior photostability, and high reliability in various applications. Goat Anti-Rabbit IgG ATTO490LS offers a large Stokes shift of 125 nm, providing superior spectral separation between excitation and emission wavelengths, making it ideal for multiplexing applications and reducing spectral overlap.
The ATTO490LS dye exhibits a distinct excitation/emission spectrum (λex: 495 nm, λem: 658 nm), ensuring compatibility with standard fluorescence equipment. This dye’s strong fluorescence signal and low background autofluorescence make it highly effective in detecting low-abundance targets and achieving precise spatial resolution, even in complex biological samples.
This antibody is preservative-free, making it suitable for sensitive applications such as live-cell imaging, FRET, and super-resolution microscopy. Its high quantum yield and minimal non-specific binding deliver consistent results across a wide range of experimental conditions.
|
Specification |
Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
Optical Properties |
λex: 495 nm, λem: 658 nm |
Properties
Form |
Lyophilized |
Nominal concentration |
2 mg/ml (after reconstitution)
The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C. |
Content |
1.0 mg Goat Anti-Rabbit IgG (H+L) ATTO490LS
1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody)
Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
Clonality |
Polyclonal |
Isotype |
IgG |
Purification notes |
Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to rabbit IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody.
Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
Shipping conditions |
Shipped at ambient temperature. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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