
Goat Anti-Rabbit IgG ATTO514 (H+L)
Antibody produced in goat, affinity purified
Cat. #: 2303-1MG
Applications
Optimized for fluorescence microscopy, flow cytometry, and other fluorescence-based applications, containing no preservatives for compatibility with sensitive assays.
Description
Product name |
Goat Anti-Rabbit IgG (H+L) ATTO514 | GXR ATTO514 |
Target species |
Rabbit |
Description |
ATTO-labeled secondary antibodies set a high standard in fluorescence labeling, combining exceptional brightness, superior photostability, and reliable performance. Goat Anti-Rabbit IgG ATTO514 is specifically designed for applications demanding precision and sensitivity.
The ATTO514 dye features a distinct excitation/emission spectrum (λex: 511 nm, λem: 532 nm), ensuring optimal compatibility with green fluorescence channels. Its high fluorescence quantum yield and photostability make it an excellent choice for detecting low-abundance targets in complex biological samples.
This antibody is preservative-free, making it suitable for live-cell imaging, super-resolution microscopy, and fluorescence resonance energy transfer (FRET) experiments. The robust fluorescence properties ensure consistent results under various experimental conditions.
|
Specification |
Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
Optical Properties |
λex: 511 nm, λem: 532 nm |
Properties
Form |
Lyophilized |
Nominal concentration |
2 mg/ml (after reconstitution with buffer) |
Content |
1.0 mg Goat Anti-Rabbit IgG (H+L) ATTO514
1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody)
Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
Clonality |
Polyclonal |
Isotype |
IgG |
Purification notes |
Column affinity purified |
Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
Shipping conditions |
Shipped at ambient temperature. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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