GTPγS | 100mM, pH 7.4

Product name

GTPγS, Synonyms: Guanosine-5'-(γ-thio)-triphosphate, Tetralithium salt, Guanosine-5'-(3-thio)-triphosphate

Product description

Adenosine-5'-(3-thio)-triphosphate (GTPγS) is used as a non-hydrolyzable analogue of GTP, to study energy storage and metabolism in vitro. Small G Proteins have a functional GTPase activity, where GTPγS is used for constitutive activation.

Overview

Suited to supplement all buffers and solutions buffered at ~pH 7.4.

Form

Lyophilized, ready-to-use.

Quantity

Powder: 100mM GTPγS, 50mM Hepes titrated with NaOH to pH 7.4. Sterile filtered before freeze-drying. Weigh into the tube 25 mg of ultrapure H₂O, corresponding to 25 µl ultrapure H₂O, to obtain a fresh 100mM stock solution.

Formula

Empirical Formula (Hill Notation) C₁₀H₁₆N₅O₁₄P₃S₃ (free acid); Molecular Weight 523.24 of the pure compound without buffer.

Purity

GTPγS  ≥ 95 % (HPLC); 5% GDP. Ultrapure buffer reagents.

Storage instructions

GTPγS is stored at –20°C upon arrival. The product will be stable in performance for at least 1 year from the date of purchase. Solubilized GTPγS can be aliquoted and stored at -70°C for use within 3 months. Thawed aliquots should be used within 1 week. Avoid refreezing.   

Shipping conditions 

At ambient temperature. Upon delivery store at -20°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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