Protein

Heavy Meromyosin (fragment of Myosin II from m. psoas)

Origin

Skeletal muscle, rabbit

Molecular mass

350 kDa

Protein description

Heavy Meromyosin obtained by limited chymotryptic cleavage of myosin II from rabbit psoas muscle has a molecular mass of 150kD. HMM consists of the active motor domain with the two head domains (the actin- and ATP- binding sites.) connected by the S2 regions and two pairs of  essential light chain (ELC) and regulatory light chain (RLC).

HMM is frequently used to determine the polarity of actin filaments in electron microscopy. 

Actin interaction

Binds to F-actin.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x 100 µg

Buffer

50mM KCl, 10mM Tris pH 7.6, 0.2mM DTT and 1% disaccharides, when reconstituted with 100 µl ultrapure water.

Purity

>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from rabbit skeletal muscle (m. psoas).

Protein concentration

A₂₇₈ =  0.6 ml/mg x cm

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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