HyQuant
Bradford assay; easy-to-use and fast one-step solution.
Cat. #: 6410-01
Description
| |
| Product name |
HyQuant Bradford Assay |
| Assay principle |
HyQuant is a one-step Bradford assay reagent for colorimetric protein quantification. Protein concentration is determined by adding Bradford reagent to samples and standards, mixing thoroughly and measuring absorbance at 595 nm. |
| Product description |
The HyQuant reagent is a ready-to-use Bradford assay solution designed for fast and easy handling. The assay can be performed in cuvettes using a spectrophotometer or in microtiter plates using a plate reader.
The workflow is straightforward: mix equal volumes of samples or standards with the assay reagent, incubate as required for color development and measure absorbance at 595 nm. Due to the dilution of sample and reagent, cuvette-based use is compatible with many common buffer substances. |
| Detection |
Colorimetric measurement at 595 nm. |
| Product availability |
Currently only available in Europe. |
Properties
| |
| Form |
Ready-to-use solution. |
| Content |
500 ml HyQuant Bradford assay reagent. |
| Assay format |
Suitable for cuvettes, micro cuvettes and microtiter plates. |
| Assay procedure |
Add HyQuant Bradford reagent to samples and standards using equal volumes. Mix thoroughly and measure absorbance at 595 nm using a spectrophotometer or plate reader. |
| Remarks |
For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Compatible Substances
| |
| Ammonium sulfate |
1.0 M |
| EDTA |
0.1 M |
| Glycine |
0.1 M |
| Glucose |
1.0 M |
| Glycerol |
5% |
| CHAPS |
0.03% |
| SDS |
0.005% |
| Sodium azide |
0.5% |
| Tris |
2.0 M |
| Triton X-100 |
0.025% |
Compatibility values are based on the supplied product information.
References
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–254.
Chial HJ, Splittgerber AG. A comparison of the binding of Coomassie brilliant blue to proteins at low and neutral pH. Anal Biochem. 1993;213:362–369.
Compton SJ, Jones CG. Mechanism of dye response and interference in the Bradford protein assay. Anal Biochem. 1985;151:369–374.
Pande SV, Murthy MSR. A modified micro-Bradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents, and lipids. Anal Biochem. 1994;220:424–426.
