Actin-Toolkit Fluorescence Microscopy ATTO390-Actin - cardiac actin
Actin-Toolkit Fluorescence Microscopy
Bovine α-cardiac Atto390-Actin
Cat. # 8820-01
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Applications
- Microscopical analysis of actin filament bundles or networks;
Description
Protein |
Atto390-Actin (α-cardiac actin) |
Origin |
Alpha-cardiac actin, bovine |
Molecular mass |
42kDa |
Toolkit description |
The Actin-Toolkit Fluorescence Microscopy with cardiac actin was developed to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In the assays, Atto390-Actin (λex 390nm, λem 479nm) is mixed with the ligand to observe the effects on either G- or F-actin. In contrast to staining with phalloidin conjugates, which can interfere with ligand binding sites on the actin molecule, no such observations were made with Atto labeled actin. Atto-Actins are validated to possess the polymerization properties of native actin. The Toolkit further contains unlabeled actin (0.5mg α-cardiac actin). A mixture of both excludes possible interference of the fluorescent actin conjugates on ligand binding sites. Several standardized experiments are described step-by-step in the handbook supplied with the kit. In combination with the actin buffers contained in the kit, error-free handling is guaranteed. The kit contains 4x100µg of α-cardiac Atto390-Actin conjugates and buffers to keep the actin functional. |
Purity |
>99% by scanning densitometry. |
Properties
Form |
Lyophilized, ready-to-use. |
Kit content |
4x100µg Atto390-Actin (lyophilized powder); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. |
Related products |
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Purification notes |
Purified from bovine cardiac muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
51005-14-2 (Actin) |
Further Information
Material and Safety Data Sheet
Table of Dyes and Light Sources
Material and Safety Data Sheet
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