Actin-Toolkit Fluorescence Microscopy ATTO594 Actin - skeletal muscle actin
Actin-Toolkit
Fluorescence Microscopy
Rabbit Skeletal Muscle ATTO594-Actin
Cat. # 8087-01
Applications
- Microscopical analysis of actin filament bundles or networks;
Description
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|
Protein |
ATTO594-Actin (α-Actin) |
Origin |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Toolkit description |
The Actin-Toolkit Fluorescence Microscopy was developed to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In the assays, ATTO594-Actin (λex 594nm, λem 627nm) is mixed with the ligand to observe the effects on either G- or F-actin. In contrast to staining with phalloidin conjugates, which can interfere with ligand binding sites on the actin molecule, no such observations were made with ATTO labeled actin. ATTO-Actins are validated to possess the polymerization properties of native actin.
The Toolkit further contains unlabeled actin (0.5mg α-skeletal muscle actin). A mixture of both excludes possible interference of the fluorescent actin conjugates on ligand binding sites. Vinculin tail - a V8 protease cleavage product of vinculin - is an efficient actin bundling vinculin domain, is supplied with the Toolkitas a bundling reference.
Several standardized experiments are described step-by-step in the handbook supplied with the kit. In combination with the actin bufferscontained in the kit, error-free handling is guaranteed. The kit contains 4x100µg of α-skeletal muscle ATTO594-Actin conjugates and buffers to keep the actin functional. |
Purity |
>99% by scanning densitometry. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Kit content |
4x100µg ATTO594-Actin (lyophilized powder); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. |
Related products |
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Purification notes |
Purified from rabbit skeletal muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
51005-14-2 (Actin) |
Further Information
Material and Safety Data Sheet
Table of Dyes and Light Sources
Material and Safety Data Sheet
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