Actin-Toolkit F-Actin Binding
α-actin from bovine cardiac muscle
Cat. #: 8100-01
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Applications
- Proof of the biological activity of a ligand by actin binding;
- Cosedimentation analysis of ligands binding to F-actin;
- Testing of actin filament bundling activity or network formation by ligands;
- Analysis of pharmacological compounds, proteins or lipids altering ligand-actin-interaction;
Description
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|
Protein |
Actin (α-cardiac Actin) |
Origin |
Cardiac muscle alpha actin, bovine |
Molecular mass |
42kDa |
Toolkit description |
Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Specific actin binding indicates that the ligand is correctly folded and structurally intact. The Actin-Toolkit F-Actin Binding is a well-established and commonly used method to characterize ligand-actin interaction: at high speed centrifugation ligands binding to individual actin filaments are co-sedimented. At low speed spinning a selective co-sedimentation of actin filament bundles or networks is achieved. Low speed assays instantly characterize ligands to induce actin suprastructures.
The Actin-Toolkit F-Actin Binding was developed to characterize the actin-binding properties of ligands (native or recombinant proteins, recombinant fragments etc.) and to support the detection even of cryptic actin-binding sites. Further suitable applications are in vitro compound analysis of the ligand-actin interactions by investigating effects of pharmacological compounds on ligand-actin complexes, as well as the introduction of further ligands (proteins, lipids etc.) to ligand-actin complexes. |
Purity |
>99% by scanning densitometry. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Kit content |
4x1 mg Actin (lyophilized powder); |
Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution. |
Related products |
For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat. #: 5000-0*). |
Purification notes |
Purified from bovine cardiac muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Further Information
Material and Safety Data Sheet
Material and Safety Data Sheet
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