Actin-Trialkit G-Actin Binding (alpha-cardiac actin)

Product name

Actin

Target species

Cardiac muscle alpha actin, bovine

Molecular mass

42kDa

Triallkit description

Binding of ligands to G-actin proves the biological activity of the ligand and identifies the ligand to be a cardiac G-actin binding protein. The assays of this kit are based on the spin-down analysis of ligands bound to G-actin. For this purpose G-actin has been conjugated to Sepharose™ beads (ActinBeads) which are ready-to-use.

ActinBeads are pelleted at low centrifugal forces and ligands bound to G-actin will be specifically co-precipitated. SDS-PAGE analysis will reveal binding of G-actin binding ligands. The Trialkit is further used for assays ranging from the determination of binding stoichiometries using gelsolin as reference, up to variation of buffer conditions to possibly optimize G-actin binding.

The kit contains 1x250µl ActinBeads (50% slurry) prepared from rabbit skeletal muscle alpha actin (Cat. # T8020-01) or bovine cardiac alpha actin (Cat. # T8200-01) and buffers to keep actin functional. The Trialkit Manual describes the use of ActinBeads. We guarantee a stable performance of the kit components when stored at -70°C upon arrival for 6 months.

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

1x250 µl ActinBeads (50% slurry, lyophilized Sepharose™ conjugated actin);
2x50 ml MonoMix (lyophilized G-actin ATP-buffer);
1x Trialkit Manual G-Actin Binding;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with ultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). 

Purification notes

Purified from bovine cardiac muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)