Vinculin (smooth muscle, turkey) - 250µg
(Actin Binding Protein)
Cat. #: 8307-02
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Description
Protein |
Vinculin |
Origin |
Smooth muscle, turkey |
Molecular mass |
116kDa |
Protein description |
Vinculin from turkey smooth muscle (gizzard) is a single chain polypeptide with a molecular weight of 116kD. Vinculin is a multiligand binding protein e.g. of focal contacts, possessing an F-actin binding domain in the tail region. |
Actin interaction |
Actin binding protein. |
Properties
Form |
Lyophilized, ready-to-use. |
Quantity per unit |
1x250 µg |
Buffer |
100mM KCl, 10mM imidazole pH 7.5, 0.5mM DTT, 0.1mM EGTA and 0.25% disaccharides, when reconstituted with 250µl ultrapure water to obtain a 1mg/ml solution. |
Purity |
>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
Purification notes |
Purified from turkey gizzard muscle. |
Protein concentration |
Determined by Biuret method. |
Storage instructions |
Vinculin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 2-3 weeks. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Reconstitution of contractile actomyosin rings in vesicles.
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N. , Elife. 2020;9:e56110. Published 2020 Jul 13. doi:10.7554/eLife.56110
Structural basis for vinculin activation at sites of cell adhesion.
Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC.
Nature. 2004 Jul 29;430(6999):583-6. Epub 2004 Jun 13.
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N. , Elife. 2020;9:e56110. Published 2020 Jul 13. doi:10.7554/eLife.56110
Structural basis for vinculin activation at sites of cell adhesion.
Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC.
Nature. 2004 Jul 29;430(6999):583-6. Epub 2004 Jun 13.
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