NEM-Myosin II (rabbit, m. psoas) - 1x200 µg
(Actin Binding Protein)
Cat. #: 8316-01
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Application
Tethering protein for TIRF microscopy, biochemical assays
Description
Protein |
NEM-Myosin |
Origin |
Skeletal muscle, rabbit |
Molecular mass |
~480kDa |
Protein description |
NEM-Myosin II is chemically inactivated fast muscle myosin II from rabbit psoas muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation. |
Actin interaction |
Binds to F-actin. |
Properties
Form |
Lyophilized, ready-to-use. |
Quantity |
1x200 µg |
Buffer |
500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution. |
Purity |
>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
Purification notes |
Purified from rabbit skeletal muscle (m. psoas). |
Protein concentration |
Determined at OD280 (0.1% = 0.53). |
Storage instructions |
Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
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