Rhodamine-Actin
alpha actin from skeletal muscle, rabbit
Cat. #: 8103-01
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Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)
Description
|
Protein |
Rhodamine Actin |
|
Origin |
Skeletal muscle alpha actin, rabbit |
|
Molecular mass |
42kDa |
|
Protein description |
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Rhodamine-Actin is chemically modified G-actin by conugation of Cys374 with tetramethylrhodamine. |
|
Conjugation ratio |
The dye/protein molar ratio was determined to be 0.5 : 1 |
Properties
|
Form |
Lyophilized, ready-to-use. |
|
Quantity per unit |
2x100 µg |
|
Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 ml ultrapure water to obtain a 1mg/ml solution. |
|
Purity |
>99% by scanning densitometry. |
|
Purification notes |
Purified by GPC from rabbit skeletal muscle. |
|
Protein concentration |
Determined by the Biuret method. |
|
Storage instructions |
Rhodamine-actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing. |
|
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
|
Remarks |
|
|
CAS no. |
Further Information
Product DataSheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
Product DataSheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
