Non-Muscle Actin - 200 µg
TOP
Non-Muscle Actin
from brain, porcine
Cat. #: 8401-01
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Description
Protein |
β/γ non-muscle actin |
Origin |
porcine brain |
Molecular mass |
42kDa |
Protein description |
Non-muscle actin is a mixture β/γ non-muscle actin isoforms each consisting of a single chain polypeptide with a molecular weight of 42kD consisting of 374 amino acids. |
Purity |
>99% by scanning densitometry. |
Properties
Form |
Lyophilized, ready-to-use. |
Quantity per unit |
200 µg |
Buffer |
2mM Tris-Cl pH 8.2, 2.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution. |
Related products |
For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). |
Purification notes |
Purified from porcine brain, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
Non-muscle actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Molecular integration of the anti-tropomyosin compound ATM-3507 into the coiled coil overlap region of the cancer-associated Tpm3.1.
Janco M, Rynkiewicz MJ, Li L, Hook J, Eiffe E, Ghosh A, Böcking T, Lehman WJ, Hardeman EC, Gunning PW.Sci Rep. 2019 Aug 2;9(1):11262. doi: 10.1038/s41598-019-47592-9.PMID: 31375704
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