Alpha-Actinin (smooth muscle) - 2x100 µg
(Actin Binding Protein)
Cat. #: 8301-01
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Description
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|
Protein |
alpha-actinin (alpha-actinin 1) |
Origin |
Smooth muscle, turkey gizzard |
Molecular mass |
~200kDa |
Protein description |
Native α-actinin 1 from smooth muscle has a molecular mass of 200kDa and forms homodimers (2x100kDa). α-Actinins belong to the spectrin superfamily and are divided in four isoforms, encoded by different genes. Isoform 2 and 3 – the so called "muscle" α-actinins are constituents of sarcomeres in the cross-striated skeletal and cardiac muscle. Isoform 1 and 4 are "non-muscle" α-actinins and present in practically every tissue. |
Actin interaction |
Actin cross linking protein. Kd actin-binding = 1-5µM. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Quantity per unit |
2x100 µg |
Buffer |
50mM NaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution. |
Purity |
>97% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
Purification notes |
Purified from turkey gizzard muscle (IEX, SEC). |
Protein concentration |
Determined UV-VIS (A280=0.1201) |
Storage instructions |
Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
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Further Information
References
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P.Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Real-time dynamics of emerging actin networks in cell-mimicking compartments.
Deshpande S, Pfohl T.; PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521. eCollection 2015.
A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.
Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.; J Cell Sci. 1995 Mar;108 ( Pt 3):857-68
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P.Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Real-time dynamics of emerging actin networks in cell-mimicking compartments.
Deshpande S, Pfohl T.; PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521. eCollection 2015.
A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.
Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.; J Cell Sci. 1995 Mar;108 ( Pt 3):857-68
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