Actin-Toolkit ELISA
α-cardiac Actin, bovine
Cat. #: 8122-01
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Applications
Description
Proteins |
Biotin-Actin (biotinylated α-cardiac actin) |
Origin |
Cardiac muscle alpha actin, bovine |
Molecular mass |
42kDa |
Toolkit description |
The Actin-Toolkit ELISA is used to analyze ligand interactions with G-Actin or F-actin by enzyme linked immunosorbant assay. Purified proteins or other ligands, semi purified ligands or crude extracts can be used for the assays. Thus the Toolkit is suited for quantitative analyses or to determine actin-binding at various concentrations e.g. with crude extracts. 2. Sandwich ELISA.: the ligand is coated to the surface of the plate and either G-Biotin-actin or F-actin is used to directly interact with the ligand. To detect Actin successfully bound to the coated ligand the anti-actin antibody (clone 2G2) is used to be detected by secondary antibodies (mouse anti IgG or mouse anti IgM - HRP or alk. Phosphatase conjugated) is used. Anti-Actin is an IgM and thus able to efficiently bind to five actin. This results in an amplification of the signal obtained by secondary antibodies. The kit contains Biotin-Actin, Actin and monoclonal anti-Actin, buffers for ELISA keeping actin functional and preventing denaturing of actin. A handbook introduces and guides through the experimental work with actin. |
Purity |
>99% by scanning densitometry. |
Properties
Form |
Lyophilized, ready-to-use. |
Kit content |
2x100µg Biotin G-Actin (lyophilized powder); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1mg/ml solution. |
Related products |
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Purification notes |
Purified from rabbit skeletal muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
51005-14-2 (Actin) |
Further Information
Material and Safety Data Sheet
Protein Sequence on NCBI
Material and Safety Data Sheet
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