Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)
Actin-Toolkit Microinjection
ATTO565-Actin, Skeletal Muscle alpha-Actin
Cat. #: 8061-01
............................................................................................................................................................................................................................................................................................................................................................................................................
Applications
Visualization of transfected actin binding proteins affecting the actin cytoskeleton;
Study of the effects by injection of pre-formed ligand-actin complexes;
Analysis of components or culturing conditions on the actin cytoskeleton;
Suitable for use with cell or tissue extracts in biochemical assays;
Description
|
|
Protein |
ATTO565-Actin |
Origin |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Toolkit description |
The Actin-Toolkit Microinjection all-in-one kit contains rhodamine conjugated α-actin to be microinjected into either normal or transfected cells. For this purpose purified actin was covalently lysine labeled at ATTO565 , leaving the polymerization properties of actin essentially unimpaired. |
Purity |
>99% by scanning densitometry. |
Properties
|
|
Form |
Lyophilized, ready-to-use. |
Kit content |
4x100µg ATTO565-Actin (lyophilized powder); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution. |
DOL |
>0.5 |
Purification notes |
Purified from rabbit skeletal muscle, GPC. |
DOL |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
|
Further Information
Material and Safety Data Sheet
{{.}}
{{/content}}{{{.}}}
{{/content}}