Ubiquitin ATTO 488 - 200µg
human, recombinant conjugate
Cat. #: 8421-01t
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Applications
Fluorescence microscopy, FRET, Fluorescence spectroscopy, Biochemical assays.
Description
|
|
Protein |
Ubiquitin-ATTO488 |
Origin |
human, recombinant |
Molecular mass |
9.2kDa |
Protein description |
Ubiquitin is a ubiquitos regulatory protein (8.5kDa) that can be conjugated to substrate proteins by subsequent actions of enzymes, such as:: E1 - class of Ubiquitin activating enzymes, E2 - class of Ubiquitin conjugating enzymes and E3- class of Ubiquitin ligases, All assays have to be optimized for the individual set up and it is recommended to start with initial concentration of Ubiquitin-ATTO488 of 0.01-0.5mM.
|
Conjugation ratio |
This product is supplied with a degree of labelling (DOL) of ~1. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Quantity |
200 µg |
Buffer |
20mM TES pH 7.4, 50mM KCl and 0.3% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution. |
Purity |
>99% by scanning densitometry. |
Purification notes |
Purified by GPC |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
Ubiquitin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ubiquitin is kept on ice and should be used within 2 weeks. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
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Further Information
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
References
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.
Glickman MH, Ciechanover A.; Physiol Rev. 2002 Apr;82(2):373-428. Review.
Structure and functions of the 20S and 26S proteasomes.
Coux O, Tanaka K, Goldberg AL.; Annu Rev Biochem. 1996;65:801-47. Review.
Characterization of the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes.
Ciechanover A, Elias S, Heller H, Ferber S, Hershko A.; J Biol Chem. 1980 Aug 25;255(16):7525-8.
The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.
Glickman MH, Ciechanover A.; Physiol Rev. 2002 Apr;82(2):373-428. Review.
Structure and functions of the 20S and 26S proteasomes.
Coux O, Tanaka K, Goldberg AL.; Annu Rev Biochem. 1996;65:801-47. Review.
Characterization of the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes.
Ciechanover A, Elias S, Heller H, Ferber S, Hershko A.; J Biol Chem. 1980 Aug 25;255(16):7525-8.
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