Anti-Myc
clone 9E10, >96% purity
Cat. # 7901-01
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Description
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Product name
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Monoclonal Anti-Myc (clone 9E10)
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Target
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Immunogen : synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
Sequence : Epitope sequence, EQKLISEEDL (aa 410-419 of human c-myc protein).
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Description
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The Myc Tag is an epitope tag, commonly used to localize gene products in many types of cultured cells, to study the topology of proteins and protein complexes. Anti-Myc is especially useful whenever no primary antibodies against the proteins of interest are available, mostly because they are less immunogenic and low abundant.
Monoclonal Anti-Myc is directed against a peptide epitope of the human proto-oncogene p62c-myc. The immunogen corresponds to amino acids 408-439 of the Leucine zipper region of human c-Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA). Anti-Myc recognizes the epitope EQKLISEEDL.
The antibody is used for the immunodetection and functional characterization of Myc and recombinant proteins containing the Myc epitope tag. It is frequently used for the detection and characterization of recombinant expressed Myc-tagged proteins in vivo and in vitro or proteins containing the epitope EQKLISEEDL. Anti-Myc facilitates protein detection in all blotting techniques and immunofluorescence & -chemistry of tagged proteins.The Myc Tag is an epitope tag, commonly used to localize gene products in many types of cultured cells, to study the topology of proteins and protein complexes. Anti-Myc is especially useful whenever no primary antibodies against the proteins of interest are available, mostly because they are less immunogenic and low abundant.
Monoclonal Anti-Myc is directed against a peptide epitope of the human proto-oncogene p62c-myc. The immunogen corresponds to amino acids 408-439 of the Leucine zipper region of human c-Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA). Anti-Myc recognizes the epitope EQKLISEEDL.
The antibody is used for the immunodetection and functional characterization of Myc and recombinant proteins containing the Myc epitope tag. It is frequently used for the detection and characterization of recombinant expressed Myc-tagged proteins in vivo and in vitro or proteins containing the epitope EQKLISEEDL. Anti-Myc facilitates protein detection in all blotting techniques and immunofluorescence & -chemistry of tagged proteins.
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Specification
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Immunofluorescence (IF) . 2-10 µg/mL
Immunohistochemistry (Paraffin) (IHC (P)) : 1:100
Immunoprecipitation (IP) : 2-5 µg
ELISA (ELISA) .: 0.1-1.0 µg/mL
Western Blot (WB) : 1-2 µg/mL
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Synonyms
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Proto-oncogene c-Myc; Transcription factor p64; Class E basic helix-loop-helix protein 39; BHLHE39; cmyc |
Properties
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Form
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Freeze-dried..
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Quantity
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200 µg
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Product content
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200 µg Anti-Myc (clone 12CA5)
0.5 ml Glycerol-PBS (Reconstitution Buffer)
Product DataSheet
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Clonality
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Monoclonal
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Isotype
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Mouse IgG1, kappa
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Storage instructions
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Store as glyecrol stock at -20°C, Avoid freeze/thaw cycles.
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Remarks
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CAS no.
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Processes.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 Dec;5(12):3610-6. PMID:3915782
