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Biotin-actin conjugates are used to investigate actin interactions in-vitro assays.
Biotin-actin from skeletal or cardiac muscle α-actin are prepared by labelling lysine residues of actin with activated Biotin-esters. The advantage of this Biotin-actin is the non-cleavable 22 angstroms spacer arm, making the protein readily available for detection.
Elisa asays, Motility assays, Microinjection, Pull-down assays...