Atto-conjugated Secondary Antibodies
New generation antibodies: Superior photo-stability
& strong fluorescence !
The quality of secondary antibodies is essential in producing superior results in immunofluorescence. Hypermol offers secondary antibodies conjugated to the novel, high quality Atto dyes for detecting primary antibodies.
Atto-labeled antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. For this reason they are suited for all kinds of immunofluorescence microscopy and mostly also for high resolution techniques.
Compared to established fluorescent antibodies, Atto antibodies show a remarkably prolonged fluorescence, as a result of the outstanding features of the dyes, as demonstrated by two examples:
1. Atto 647N fluoresces in aqueous solution twice as strong as Cy5TM.
2. under identical conditions Atto 647N lasts up to 100 times longer than dyes like Cy5TMand Alexa 647TM.
Atto-conjugated IgGs from Hypermol are affinity purified and quality-control tested by UV-VIS spectroscopy and immunocytochemistry. They come as convenient kits with a well characterized, epitope mapped actin antibody for reference, double staining or ligand analysis, with a detailed description in the product documentation of each kit.