Jasplakinolide - 500µM
Jasplakinolide
Jaspamide; NSC 613009. Cell-permeant actin-stabilizing marine cyclodepsipeptide for induction of actin polymerization and inhibition of F-actin depolymerization.
Applications
| Applications | Induction of actin polymerization, stabilization of actin filaments and inhibition of F-actin depolymerization in biochemical and cell-based cytoskeleton assays. |
| Recommended use | Recommended when stable actin filaments are required, for example in assays investigating actin filament stabilization, filament turnover, actin polymerization, cytoskeletal remodeling or comparison of actin-targeting compounds. |
| Typical workflows | Used in in vitro and cell-based assays to stabilize F-actin, reduce filament depolymerization, promote actin nucleation and modulate the actin cytoskeleton in living cells or biochemical actin systems. |
Description
| Product name | Jasplakinolide |
| Synonyms | Jaspamide; NSC 613009. |
| Product description | Jasplakinolide is a macrocyclic peptide natural compound isolated from the marine sponge Jaspis johnstoni. It binds to actin filaments and inhibits their depolymerization by interaction at the interface of adjacent actin subunits within the filament. Jasplakinolide is a potent inducer of actin polymerization in vitro because it promotes actin filament nucleation. It has high affinity for F-actin and lower affinity for actin monomers. Binding of Jasplakinolide strongly reduces the rate constant for dissociation of actin monomers from filament ends, thereby stabilizing filament structure and supporting workflows requiring persistent actin filaments. |
| Mechanism | Jasplakinolide stabilizes F-actin by binding to actin filaments, reducing monomer dissociation from filament ends and inhibiting actin filament depolymerization. It also promotes actin nucleation and can shift actin dynamics toward polymerized filamentous actin. |
| Functional notes | Jasplakinolide competes with phalloidin for actin binding. It is reported to inhibit the ATPase activity of F-actin and to stabilize filament structure by strongly reducing monomer dissociation, an effect associated with trapping of ADP within actin filaments. |
| Overview | Cell-permeant actin-stabilizing compound; promotes actin polymerization; inhibits F-actin depolymerization; stabilizes actin filaments; suitable for actin cytoskeleton modulation in biochemical and live-cell assays. |
Properties
| Form | Dry compound, ready-to-use after reconstitution in DMSO. |
| Stock solution | To prepare a 500 µM stock solution, add 30 µl DMSO. Alternatively, add 150 µl DMSO to prepare a 100 µM stock solution. |
| Application note | 150 µl of a 100 µM Jasplakinolide solution stabilizes 2 mg actin at a 1:1 molar ratio. |
| Typical working concentration | Jasplakinolide is used for in vitro and in vivo assays at working concentrations from 50 nM to 5 µM. Incubation times range from 2 minutes to 24 hours. The optimal concentration and incubation time should be determined experimentally for each assay system. |
| Live-cell use | For live-cell assays, Jasplakinolide can be added to a variety of cell culture media, including complete media containing serum and antibiotics, provided that the final solvent concentration and compound concentration are compatible with the cell type and assay endpoint. |
| Solvent | DMSO. |
| CAS no. | 102396-24-7 |
| Storage instructions | Store according to the supplied storage information. Protect from light and moisture. Avoid repeated freeze-thaw cycles after reconstitution. |
| Shipping conditions | At ambient temperature, unless otherwise specified in the product documentation. Store according to the supplied storage information upon delivery. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
Further Information
Product DataSheet
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Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
