Jasplakinolide - 500µM
Jasplakinolide
Jaspamide, NSC 613009
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Jasplakinolide also inhibits the ATP-ase activity of F-actin. Binding arrests actin monomers in a specific G-actin state and stabilizes filament structure by strongly reducing the rate constant for monomer dissociation, an event associated with the trapping of ADP.
Jasplakinolide are used for in vitro and in vivo assays at working concentrations from 50 nM to 5 µM. Incubation times range 2 minutes to 24 hours.
On live cells, jasplakinolide can be added to a variety of cell culture media (complete with serum and antibiotics).
The optimal concentration and incubation time has to be determined by each user experimentally.
Units
Dry component, reconstitute with DMSO.
To prepare a 500 µM stock solution add 30 µl DMSO.
Alternatively add 150 µl to prepare a 100 µM stock solution.
► 150µl of a 100µM solution stabilize 2mg actin at a 1 : 1 molar ratio.
Cas no.: 102396-24-7
Product DataSheet
Jaspamide, NSC 613009
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Applications
Induces actin polymerization;
Stabilization of actin filaments, resp. Inhibition of actin depolymerization;
Description
Jasplakinolide binds to actin filaments and inhibits their depolymerization by specific interaction at the interface of adjacent monomers of the filaments. Jasplakinolide is a macrocyclic peptide isolated from the marine sponge Jaspis johnstoni. It is a very potent inducer of actin polymerization in vitro, since it promotes actin filament nucleation. Notably Jasplakinolide competes with phalloidin for actin binding with a Kd = 15 nM.
Jasplakinolide has a high affinity for actin filaments and lower affinity for actin monomers. Binding of Jasplakinolide leads to a decrease in the rate constant for the dissociation of actin monomers from the filament ends. This results in a stabilization of filaments, used in many assays where stable actin filaments are demanded.
Jasplakinolide has a high affinity for actin filaments and lower affinity for actin monomers. Binding of Jasplakinolide leads to a decrease in the rate constant for the dissociation of actin monomers from the filament ends. This results in a stabilization of filaments, used in many assays where stable actin filaments are demanded.
Jasplakinolide also inhibits the ATP-ase activity of F-actin. Binding arrests actin monomers in a specific G-actin state and stabilizes filament structure by strongly reducing the rate constant for monomer dissociation, an event associated with the trapping of ADP.
Jasplakinolide are used for in vitro and in vivo assays at working concentrations from 50 nM to 5 µM. Incubation times range 2 minutes to 24 hours.
On live cells, jasplakinolide can be added to a variety of cell culture media (complete with serum and antibiotics).
The optimal concentration and incubation time has to be determined by each user experimentally.
Dry component, reconstitute with DMSO.
To prepare a 500 µM stock solution add 30 µl DMSO.
Alternatively add 150 µl to prepare a 100 µM stock solution.
► 150µl of a 100µM solution stabilize 2mg actin at a 1 : 1 molar ratio.
Cas no.: 102396-24-7
Product DataSheet 
