KMEI buffer is a multipurpose buffer used e.g. as a protein buffer, for dilution, and storage of cytoskeletal proteins. The buffer is frequently in TIRFM and fluorimetry to polymerize actin. The buffer conditions are suited for proteins stored at pH 7.0 - 7.5.
Overview
Essential buffer to:
use for sample preparation;
dilute protein stocks;
storage and preparation of glycerol stocks;
Properties
Form
Liquid, ready-to-use.
Quantity
4x1.0 ml 10x stock
Buffer
The 10x stock solution contains 0.5M KCl, 20mM MgCl2, 10mM EDTA and 0.1M Imidazole pH 7.1. The buffer is added 1 : 10 v/v to actin solutions.
Purity
Ultrapure reagents.
Storage instructions
KMEI Buffer is stored at RT upon arrival and will be stable in performance for at least 6 months from the date of purchase after reconstitution. Protect from light.
Shipping conditions
At ambient temperature in light protective tubes. Upon delivery store at RT.
Remarks
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
CAS no.
Further Information
Product DataSheet
Material and Safety Data Sheet
Buffers from Hypermol are made of purest reagents in Milli-Q™ water, as described in our publications.
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