Monoclonal anti Gelsolin

Product name

Monoclonal Anti-Gelsolin (clone 2E13)

Target species

Porcine cytoplasmic gelsolin

Description

Monoclonal anti gelsolin (99% purity) is an epitope mapped antibody that recognizes cytoplasmic gelsolin in humans and other mamals, and birds. Anti gelsolin 2E12 can be used to differetiate between cytoplasmic and plasma gelsolin. The antibody does not recognize plasma gelsolin, probably because the epitope is hidden by the N-terminal extension of the plasma gelsolin isoform.

As immunogen for monoclonal anti gelsolin2E12 cytoplasmic smooth muscle gelsolin was used and epitope mapping localized the following sequence (Fock et al., 2005):

YGDFFTGDAYVILKTLGNECSQDESAAAI.

Monoclonal anti gelsolin has been validated in immunofluorescence, immunoblot and ELISA

Specification

Immunofluorescence: 1:100-1:500, 
Immunoblot: 1:500-1500.

ELISA: 1:5000

Optical properties

Form

Freeze-dried.

Quantity

250 µg

Content


Purity

Buffer recommendation

250 µg monoclonal Anti-Actin (clone 2E12)
Product documentation

99% pure (IEX-chromatography)

50% glycerol, 3% BSA, 0.01 M sodium phosphate, 0.1 M 
sodium chloride, 5 mM sodium azide, pH 7.4.

Clonality

Monoclonal

Isotype

IgG1

Storage instructions

Store as glyecrol stock at -20°C, Avoid freeze/thaw cycles.

Remarks

CAS no.

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Processes.