Myosin II
(Actin Binding Protein)
Cat. #: 8306-01
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Description
|
Protein |
Myosin II |
|
Origin |
Skeletal muscle, rabbit (m.psoas) |
|
Molecular mass |
~480kDa |
|
Protein description |
Fast muscle myosin II from rabbit psoas muscle is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation. Myosin II has two identical head domains, where the actin binding sites are localized. It consists of two heavy chains (each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains). |
|
Actin interaction |
Binds to F-actin. |
Properties
|
Form |
Lyophilized, ready-to-use. |
|
Quantity per unit |
1,0 mg |
|
Buffer |
500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 1,0 ml ultrapure water to obtain a 1mg/ml solution. |
|
Purity |
>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
|
Purification notes |
Purified from rabbit skeletal muscle (m. psoas). |
|
Protein concentration |
Determined by Biuret method with actin (rabbit skeletal muscle) as reference. |
|
Storage instructions |
Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
|
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
|
Remarks |
For coated or filamentous myosin II MotorMix I (Cat. #: 5502-01) or PolyMix (Cat. #: 5000-0*) can be used.MotorMix II (Cat. #: 5503-01) is required for soluble myosin II. |
|
CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
