NEM-Myosin II (rabbit, m. psoas) - 1x200 µg
NEM-Myosin II
(Actin Binding Protein)
Cat. #: 8316-01
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Application
Tethering protein for TIRF microscopy, biochemical assays
Description
Protein |
NEM-Myosin |
Origin |
Skeletal muscle, rabbit |
Molecular mass |
~480kDa |
Protein description |
NEM-Myosin II is chemically inactivated fast muscle myosin II from rabbit psoas muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation. |
Actin interaction |
Binds to F-actin. |
Properties
Form |
Lyophilized, ready-to-use. |
Quantity |
1x200 µg |
Buffer |
500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution. |
Purity |
>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
Purification notes |
Purified from rabbit skeletal muscle (m. psoas). |
Protein concentration |
Determined at OD280 (0.1% = 0.53). |
Storage instructions |
Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Kim HR, Kwon MS, Lee S, Mun Y, Lee KS, Kim CH, Na BR, Kim BNR, Piragyte I, Lee HS, Jun Y, Jin MS, Hyun YM, Jung HS, Mun JY, Jun CD.
Sci Rep. 2018 Apr 3;8(1):5503. doi: 10.1038/s41598-018-23816-2.
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