ATTO532-Actin for TIRFM (non-muscle) - 2x50µg

Protein

ATTO532-Actin

Origin

Non-muscle β/γ actin, porcine

Molecular mass

42kDa

Protein description

Actin from non-muscle sources is mixture of  β/γ non-muscle actin isoforms. Both isoforms are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. ATTO532-Actin is chemically modified G-actin with an ATTO532 fluorescent group covalently bound to lysine residues. 

ATTO532-Actin (λex 532nm, λem 553nm) possesses the polymerization properties of native G-actin. 

Conjugation ratio

This product is supplied with a degree of labelling (DOL)  of ~0.3 and is thus ready-to-use for TIRFM and other high- or superresolution imanging techniques.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50 µg

Buffer

2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from porcine brain.

Protein concentration

Determined by the Biuret method.

Storage instructions

ATTO532-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin is kept on ice and should be used within 1 week. Avoid refreezing.   

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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