ATTO647-Actin for TIRFM (non-muscle actin) - 2x50µg
ATTO647-Actin for TIRFM
Superresolution-optimized fluorescent porcine brain non-muscle β/γ-actin; lysine-labelled with ATTO647.
Applications
| Applications | Superresolution-optimized, polymerization-competent fluorescent porcine brain non-muscle β/γ-actin for TIRFM, high- and super-resolution fluorescence microscopy, FRET, filament tracking, fluorescence spectroscopy and biochemical actin assays. |
Description
| Product name | ATTO647-Actin for TIRFM |
| Protein | Porcine brain non-muscle β/γ-actin. |
| Molecular mass | 42 kDa. |
| Product description | ATTO647-Actin for TIRFM is chemically modified porcine brain non-muscle G-actin carrying covalently attached ATTO647 fluorescent groups on lysine residues. Non-muscle actin from brain is supplied as a β/γ-actin mixture; both actin isoforms are single-chain polypeptides of approximately 42 kDa consisting of 375 amino acids. The product is prepared as a superresolution-optimized fluorescent actin probe with an adjusted degree of labelling to support bright far-red fluorescence detection while preserving the polymerization properties of native G-actin. It is designed for TIRF microscopy and other fluorescence-based actin assays requiring polymerization-competent labelled actin. |
| Fluorophore | ATTO647. |
| Spectral properties | Excitation maximum: 647 nm; emission maximum: 669 nm. |
| Labelling chemistry | ATTO647 is covalently attached to lysine residues of porcine brain non-muscle G-actin. |
| Degree of labelling | This product is supplied with an optimized degree of labelling (DOL) of approximately 0.3 to support superresolution and TIRF microscopy applications. |
| Overview | Superresolution-optimized fluorescent porcine brain non-muscle β/γ-actin; ATTO647-labelled on lysine residues; polymerization-competent; suitable for TIRFM, high- and super-resolution fluorescence microscopy, FRET, filament tracking and biochemical actin assays; supplied lyophilized in actin-compatible buffer; 2 × 50 µg package variant. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution. |
| Quantity per unit | 2 × 50 µg. |
| Reconstitution | Reconstitute each 50 µg tube with 50 µl ultrapure water to obtain a 1 mg/ml ATTO647-Actin solution. |
| Buffer | 2 mM Tris-HCl pH 8.2, 1 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2 and 0.3% disaccharides after reconstitution with ultrapure water to obtain a 1 mg/ml solution. |
| Purity | >99% by scanning densitometry. |
| Purification notes | Purified by gel permeation chromatography from porcine brain. |
| Protein concentration | Determined by UV absorbance at 290 nm (A290 = 0.63 for 1 mg/ml actin) with fluorophore-specific correction factor applied. |
| Storage instructions | ATTO647-Actin stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized product should be kept protected from light and on ice and used within 1 week. Avoid refreezing of actin. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are prepared with ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Download product datasheet for ATTO647-Actin for TIRFM.
Material and Safety Data Sheet
MSDS / SDS document for ATTO-labelled actin products.
Protein Sequence on NCBI
Reference sequence information for porcine non-muscle actin.
Hide ATTO dyes & spectral properties
Open by default; click to collapse. Hover/tap a dye for excitation hints.
ATTO dyes – spectral overview (λex / λem)
Hover/tap a dye to see details incl. possible excitation source.
