ATTO647-Actin for TIRFM (non-muscle actin) - 2x50µg

Protein

Atto647-Actin

Origin

Non-muscle β/γ -actin, porcine brain

Molecular mass

42kDa

Protein description

Actin from non-muscle sources is a mixture of β/γ non-muscle actin isoforms. Both are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified G-actin with an Atto647 fluorescent group covalently bound to lysine residues. Atto647-Actin (λ_ex 647nm, λ_em669nm) possesses the polymerization properties of native G-actin. 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) ~0.3 and is thus  ready.-to-use for TIRFM and other high- or super-resolution imaging techniques.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50 µg

Buffer

2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from porcine brain.

Protein concentration

Determined by the Biuret method.

Storage instructions

Atto647-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized product is kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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