Phalloidin - 100µM
Phalloidin
from Amanita phalloides. Bicyclic heptapeptide for stabilization of F-actin and inhibition of actin filament depolymerization.
Applications
| Applications | Stabilization of actin filaments and inhibition of F-actin depolymerization in biochemical actin assays. |
| Recommended use | Recommended when stable actin filaments are required, for example for filament stabilization, polymerization-promoting assay conditions or mechanistic comparison with actin-destabilizing compounds. |
| Typical workflows | Used to stabilize filamentous actin, reduce subunit dissociation from filament ends and support assay conditions in which preformed or polymerizing actin filaments must remain stable during handling or analysis. |
Description
| Product name | Phalloidin |
| Source | Amanita phalloides. |
| Product description | Phalloidin is a bicyclic heptapeptide from Amanita phalloides that binds with high specificity to filamentous actin. It stabilizes F-actin by binding at the interface between adjacent actin subunits within the filament and strongly reduces subunit dissociation from filament ends. By locking neighboring actin subunits together, Phalloidin inhibits actin filament depolymerization and shifts actin dynamics toward stable filamentous actin. It is therefore widely used as a biochemical tool when stable actin filaments are required. |
| Mechanism and functional profile | Phalloidin acts primarily on filamentous actin. It binds at the interface between neighboring actin subunits within F-actin, stabilizes the filament structure and strongly reduces the dissociation rate of actin subunits from filament ends. This high-affinity interaction inhibits actin filament depolymerization, promotes stable actin polymers under suitable assay conditions and makes Phalloidin a useful reagent for biochemical assays requiring persistent filamentous actin. Phalloidin has also been reported to inhibit the ATPase activity of F-actin. |
| Overview | F-actin stabilizing peptide; inhibits filament depolymerization; promotes stable actin polymers; suitable for biochemical actin assays requiring persistent filamentous actin. |
Properties
| Form | Dry component, ready-to-use after reconstitution. |
| Stock solution | 100 µM in 500 µl after reconstitution. |
| Application note | 500 µl of a 100 µM Phalloidin solution stabilize 2 mg actin at a 1:1 molar ratio. |
| Chemical class | Bicyclic heptapeptide; phallotoxin. |
| Source | Amanita phalloides. |
| Target structure | Filamentous actin, F-actin. |
| Biological activity | Stabilizes actin filaments by inhibiting depolymerization and reducing subunit dissociation from filament ends. |
| Handling note | Use appropriate laboratory precautions for toxic actin-binding compounds. Protect reconstituted material from unnecessary repeated freeze-thaw cycles and follow the product data sheet and safety data sheet for handling and storage. |
| Storage instructions | Reconstitute upon arrival and store according to the product data sheet. Protect from light and avoid repeated freeze-thaw cycles after reconstitution. |
| Shipping conditions | Use the shipping and storage conditions specified in the supplied product documentation. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
Further Information
Product DataSheet
Material and Safety Data Sheet
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Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.