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ATTO390-Actin (alpha-Actin, skeletal muscle rabbit) - 5x100µg

Cat. #:
8152-02
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
5x100 µg


ATTO390-Actin
α-actin from rabbit skeletal muscle  
 

Cat. #: 8152-02

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Applications

Fluorescence microscopy, FRET, Fluorescence spectroscopy, Biochemical assays.

 

Description

   

Protein

ATTO390-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. ATTO390-Actin is chemically modified G-actin with an ATTO390 fluorescent group covalently bound to lysine residues.

ATTO390-Actin (
λex 390nm, λem 479nm) possesses the polymerization properties of native G-actin. 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. The precise DOL is stated in the DataSheet supplied with the product.



Properties

   

Form

Lyophilized, ready-to-use.

Quantity per unit

5x100 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

ATTO-Actins are stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin is kept on ice and should be used within 1 week. Avoid refreezing.   

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 




Further Information


Product DataSheet

Material and Safety Data Sheet

Table of Dyes and Light Sources 

Protein Sequence on NCBI
 




References


Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation.

Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.

Nat Cell Biol. 2016 Sep;18(9):941-53. doi: 10.1038/ncb3402. Epub 2016 Aug 22.