Actin-Trialkit Fluorescence Microscopy Rhodamine-Actin (skeletal muscle actin)
Actin-Trialkit Fluorescence Microscopy
Rhodamine-Actin, Skeletal Muscle Rabbit
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Applications
Visualization of ligand-induced actin polymerization;
Microscopical analysis of actin filament bundles or networks;
Description
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Product name |
Actin |
Target species |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Triallkit description |
The Actin-Trialkit Fluorescence Microscopy is used to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In combination with the actin buffers contained in the Trialkit, error-free handling of Rhodamine-Actin will be achieved. |
Purity |
>99% by scanning densitometry. |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Kit content |
1x100 µg Rhodamine-Actin (lyophilized); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with ultrapure water to obtain a 1mg/ml solution. |
Related products |
For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). |
Purification notes |
Purified from rabbit skeletal muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
51005-14-2 (Actin) |
Further Information
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