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Biuret Reagent

Cat. #:
6302-01
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Biuret
Biuret reagent
Ready-to-use concentrate


Cat. #: 6302-01
 

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Description

The Biuret assay is a reliable method to determine the concentration of soluble and insoluble proteins by complexation of peptide bonds with copper(II)ions. In principle, the assay is performed in an alkaline environment by addition of  3% NaOH (final concentration) to the protein solution. Thus also insoluble proteins can be used to determine the protein concentration, in contrast to e.g. Bradford type assays.
The assay is commonly used, when larger sample volumes are present, due to the relatively low sensitivity (~0.1-5mg/ml).  
The protein concentration is determined by colorimetric measurement at 540-550nm.


Biuret complex


Content

50ml Biuret solution (standardized solution)

This quantity is sufficient to make ~1.600 assays in half-micro cuvettes (630µl) or >8.000 assays using micro cuvettes or microtiter plates

To be prepared: 6% NaOH solution 



Product use

Mix 60µl of the protein solution (0.5mg/ml) with 60µl NaOH (6%) and add 6µl of Biuret. After 20-30min the samples are measured at 540-550nm using Microcuvetts or Elisa reader.

Reference: sample buffer or H2O.


Sensitivity


> 0.1mg/ml 

 
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