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Pyrene Actin 10% (rabbit skeletal muscle alpha actin) - 1.0 mg

Cat. #:
8102-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
1.0 mg
Units:
1x
5x ( + 353,00 EUR )


Pyrene Actin (10%)

α-actin from rabbit skeletal muscle

Cat. #: 8102-01

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Applications
Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)

 

Description

   

Protein

Pyrene Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene-Actin is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.

The actin polymerization can be induced simply by mono- or divalent cations (in-vitro conditions) and the actin kinetic can then be measured fluorometrically. Fluorometry with Pyrenyl-Actin is standard  method  to study the effect of ligands on actin kinetics.

Conjugation ratio

Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio of 1. 



Properties

   

Form

Lyophilized, ready-to-use.

Quantity per unit

1x1 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Pyrene actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

 

CAS no.

 




Further Information


Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI




References
 

Cysteine-rich protein 2 accelerates actin filament cluster formation.
Kihara T, Sugimoto Y, Shinohara S, Takaoka S, Miyake J.; PLoS One. 2017 Aug 16;12(8):e0183085. doi: 10.1371/journal.pone.0183085. eCollection 2017.
 

Cooperative effects of tropomyosin on the dynamics of the actin filament.
Khaitlina S, Tsaplina O, Hinssen H.; FEBS Lett. 2017 Jul;591(13):1884-1891. doi: 10.1002/1873-3468.12700. Epub 2017 Jun 11.



 
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