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GST-VCA (human recombinant WAVE VCA-domain, GST-tagged) - 250µg

Cat. #:
8416-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
250 µg

 


GST-VCA
Human recombinant WAVE VCA-domain, GST-tagged


Cat. #: 8416-01  

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Description

 

 

Protein

GST-VCA

Origin

recombinant, human

Molecular mass

~37.5kDa

Protein description

The VCA-domains of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous) activate in vitro the nucleation ability of Arp 2/3, which is itself a constitutively inactive complex.

The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli, purified on GST-Sepharose further column purified by gel filtration. 

Actin interaction

Activator of the Arp 2/3 protein complex (Cat. #: 8413-01).  



Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

1x250 µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 250 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity

>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli, purified on GST-Sepharose further column purified by gel filtration. The protein can detected with anti-GST antibodies using suited immunological detection systems.

Protein concentration

Determined at OD280 (0.1%=1.25)

Storage instructions

GST-VCA is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).  Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 




Further Information



Product DataSheet

Material and Safety Data Sheet

Protein Structures on RCSB (WAVE)

 

 
References
 

Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.

EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.


Structure and control of the actin regulatory WAVE complex.

Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK.

Nature. 2010 Nov 25;468(7323):533-8. doi: 10.1038/nature09623.

 

 

 
 

 

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