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Cofilin (non-muscle cofilin) - 2x50 µg

Cat. #:
8419-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg




Cofilin
(Actin Binding Protein)

Cat. #: 8419-01  
..............................................................................................................................................................................................................................................................................................................................................
 


Description

 

 

Protein

Cofilin (synonym: n-cofilin , cofilin-1)

Origin

non-muscle isoform, murine recombinant (untagged)

Molecular mass

18.5kDa

Protein description

Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics. Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.

Actin interaction

Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio. 



Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50 µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity

>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Cofilin was  expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. 

Protein concentration

Determined by OD280 (0.1%=0.778)

Storage instructions

Cofilin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).  Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 




Further Information

Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI (human non muscle)

Protein Structures on RCSB (human non muscle)




 

References
 

Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.

 

Solution structure of human cofilin: actin binding, pH sensitivity, and relationship to actin-depolymerizing factor.

Pope BJ, Zierler-Gould KM, Kühne R, Weeds AG, Ball LJ.

J Biol Chem. 2004 Feb 6;279(6):4840-8. Epub 2003 Nov 18.

 

 
 
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