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Thymosin beta-4 (human recombinant) - 2x50µg

Cat. #:
8422-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
2x50 µg

Thymosin beta-4 
(Actin Binding Protein)


Cat. #: 8422-01 

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Description

   

Protein

Thymosin beta-4 (Synonyms: T beta 4, Fx , TB4X, PTMB4, TMSB4)

Origin

recombinant, human recombinant - untagged.

Molecular mass

5kDa

Protein description

Thymosin is a hormone secreted from the thymus. The predominant form of thymosin, thymosin b4, is a member of a highly conserved family of actin monomer sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin bound pool and F-actin.

Actin interaction

The predominant form of thymosin, thymosin b4, is a member of a highly conserved family of actin monomer sequestering proteins. b-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin bound pool and F-actin.


Properties

   

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50µg

Buffer

5mM Tris pH 8.1, 0.1mM DTT and 0.2% sucrose, when reconstituted with 50µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity 


>98% by RT-HPLC.

Purification notes

Thymosin beta-4 protein was expressed in E. coli and further purified by Affinity Chromatography (Polyproline column). The pure protein was yielded by gel filtration. 

Protein concentration

Determined by by RT-HPLC.

Storage instructions

Profilin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 




Further Information


Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI

Protein Sequences and Structures on RCSB

   
 

References

Structure of actin binding proteins: insights about function at atomic resolution.
Pollard TD, Almo S, Quirk S, Vinson V, Lattman EE.; Annu Rev Cell Biol. 1994;10:207-49. Review.

Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells.
Carlsson L, Nyström LE, Sundkvist I, Markey F, Lindberg U.; J Mol Biol. 1977 Sep 25;115(3):465-83. 



 

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