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Anti-Mouse IgG F(ab) ATTO647N (H+L)

Cat. #:
2118-250UG
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
250 µg


Fluorescent Antibody Kit



Anti-Mouse IgG F(ab) ATTO647N 
Antibody produced in goat


Cat. #: 2118-250UG

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Description

   

Product name

Anti-Mouse IgG F(ab) ATTO647N 

Target species

Mouse

Description

ATTO-labeled F(ab) fragments belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG F(ab) ATTO647N is specifically suited for high resolution micoscopy, due to the high photostabilty and strong fluorescence quantum yield.


Conjugates of F(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access;
- prevention of interactions with Fc receptor-bearing membranes due to the absence of the Fc region in Fab fragments;

Specification

Goat anti-mouse IgG F(ab) ATTO647N (H+L), Immunofluorescence: 1:300-1:500, Degree of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence. 

For further information please see DataSheet. 

Optical properties

λex 645nm, λem 669nm

 
Properties

   

Form

Lyophilized

Nominal concentration

2 mg/ml

Content

 Buffer

0.25mg Anti-Mouse IgG (Fab) ATTO647N
1 ml Glycerol buffer
Product documentation

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified, IEX chromatography after papain cleavage.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

CAS no.

 




Further Information

 Product DataSheet 

Material and Safety Data Sheet

Table of Dyes and Light Sources

  Catalogue: ATTO Secondary Antibodies
 



 
References

Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.



αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.



Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.