Anti-Mouse IgG F(ab')2 ATTO647N (H+L)

Product name

Anti-Mouse IgG F(ab')2  ATTO647N 

Target species

Mouse

Description

ATTO-labeled F(ab')2 fragments belong to the next generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG F(ab')2 ATTO647N is specifically suited for high resolution micoscopy, due to the high photostabilty and strong fluorescence quantum yield.

Conjugates of F(ab')2 fragments are preferable to whole antibody conjugates, since they do not interact with Fc receptor-bearing membranes, due to the absence of the antibody Fc region.

Specification

Goat anti-mouse IgG F(ab')2 ATTO647N (H+L), Immunofluorescence: 1:300-1:1000, Degree of labeling (DOL) : >2-5, Unconjugated dye ≤5% of total fluorescence. 

For further information please see DataSheet. 

Optical properties

λex 645nm, λem 669nm

Form

Lyophilized.

Nominal concentration

2 mg/ml

Content

Buffer

0.5mg Anti-Mouse IgG F(ab')2 ATTO647N
1 ml Glycerol buffer
Product documentation

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Affinity purified IgG, SEC purified after pepsin cleavage.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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