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TMR-Actin (alpha-actin, rabbit skeletal muscle) - 500 µg

Cat. #:
8108-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
500 µg
 


TMR-Actin 

α-actin from rabbit skeletal muscle

Cat. #: 8108-01

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Description

 

 

Protein

TMR-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. TMR-Actin is chemically modified G-actin, carrying a tetramethlyrhodamine group covalently bound to Cys374 at the C-term of the molecule, rendering the actin unpolymerizable.

Conjugation ratio

TMR-Actin is supplied with a DOL=0.9-1 (DOL -Degree of labelling). 



Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity 

per unit

1x0.5 mg

Buffer

 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 0.5 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

TMR-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

 

CAS no.

 

 

 

 

Further Information


Product DataSheet

Material and Safety Data Sheet

Protein Sequence on NCBI



References
 

Exploring the stability limits of actin and its suprastructures.
Rosin C, Erlkamp M, Ecken JV, Raunser S, Winter R.

Biophys J. 2014 Dec 16;107(12):2982-2992. doi: 10.1016/j.bpj.2014.11.006.


 


 
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